|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Monkey|
|Dilution||IHC-P (2.5-3.75 µg/ml), WB (0.05-0.2 µg/ml),|
|Other Names||NEDD8-conjugating enzyme UBE2F, 6.3.2.-, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, Ubiquitin-conjugating enzyme E2 F, UBE2F, NCE2|
|Target/Specificity||Human UBE2F / UBE2F.|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||UBE2F Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.|
|Tissue Location||Widely expressed (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Gladysheva T.,et al.Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Huang D.T.,et al.Mol. Cell 33:483-495(2009).
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