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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   UBE2F Antibody (C-Terminus)   

UBE2F Antibody (C-Terminus)

Goat Polyclonal Antibody

     
  • WB - UBE2F Antibody (C-Terminus) ALS14578
    UBE2F antibody (0.05 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q969M7
Reactivity Human, Mouse, Monkey
Host Goat
Clonality Polyclonal
Calculated MW 21kDa
Dilution IHC-P (2.5-3.75 µg/ml), WB (0.05-0.2 µg/ml),
Additional Information
Gene ID 140739
Other Names NEDD8-conjugating enzyme UBE2F, 6.3.2.-, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, Ubiquitin-conjugating enzyme E2 F, UBE2F, NCE2
Target/Specificity Human UBE2F / UBE2F.
Reconstitution & Storage Store at -20°C. Minimize freezing and thawing.
PrecautionsUBE2F Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2F
Synonyms NCE2
Function Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Tissue Location Widely expressed (at protein level).
Research Areas
Citations (0)

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Background

Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

References

Gladysheva T.,et al.Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Huang D.T.,et al.Mol. Cell 33:483-495(2009).

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$ 395.00
Cat# ALS14578
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