CSRP3 Antibody (Internal)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | P50461 |
Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Horse, Bovine, Dog |
Host | Goat |
Clonality | Polyclonal |
Calculated MW | 21kDa |
Dilution | ELISA (1:32000), IHC-P (2.5-3.75 µg/ml), WB (0.01-0.03 µg/ml) |
Gene ID | 8048 |
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Other Names | Cysteine and glycine-rich protein 3, Cardiac LIM protein, Cysteine-rich protein 3, CRP3, LIM domain protein, cardiac, Muscle LIM protein, CSRP3, CLP, MLP |
Target/Specificity | Human CSRP3. |
Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
Precautions | CSRP3 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CSRP3 |
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Synonyms | CLP, MLP |
Function | Positive regulator of myogenesis. Acts as a cofactor for myogenic bHLH transcription factors such as MYOD1, and probably MYOG and MYF6. Enhances the DNA-binding activity of the MYOD1:TCF3 isoform E47 complex and may promote formation of a functional MYOD1:TCF3 isoform E47:MEF2A complex involved in myogenesis (By similarity). Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation (By similarity). The role in regulation of cytoskeleton dynamics by association with CFL2 is reported conflictingly: Shown to enhance CFL2-mediated F-actin depolymerization dependent on the CSRP3:CFL2 molecular ratio, and also shown to reduce the ability of CLF1 and CFL2 to enhance actin depolymerization (PubMed:19752190, PubMed:24934443). Proposed to contribute to the maintenance of muscle cell integrity through an actin-based mechanism. Can directly bind to actin filaments, cross-link actin filaments into bundles without polarity selectivity and protect them from dilution- and cofilin- mediated depolymerization; the function seems to involve its self- association (PubMed:24934443). In vitro can inhibit PKC/PRKCA activity (PubMed:27353086). Proposed to be involved in cardiac stress signaling by down-regulating excessive PKC/PRKCA signaling (By similarity). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:P50463}. Cytoplasm. Cytoplasm, cytoskeleton Cytoplasm, myofibril, sarcomere, Z line Cytoplasm, myofibril, sarcomere Note=Nucleocytoplasmic shuttling protein. Mainly cytoplasmic. In the Z line, found associated with GLRX3 (By similarity) {ECO:0000250|UniProtKB:P50462, ECO:0000250|UniProtKB:P50463} |
Tissue Location | Cardiac and slow-twitch skeletal muscles. Isoform 2 is expressed in striated muscle. Isoform 2 is specifically expressed at higher levels in patients with neuromuscular diseases, such as limb- girdle muscular dystrophy 2A (LGMD2A), Duchenne muscular dystrophy (DMD) and dermatomyositis (PubMed:24860983) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation (By similarity).
References
Fung Y.W.,et al.Genomics 28:602-603(1995).
Medvedev A.,et al.Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
Yasunaga S.,et al.Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
Chen K.H.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Schallus T.,et al.FEBS Lett. 583:1017-1022(2009).
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