SETD7 / SET7 Antibody (aa131-145)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
---|---|
Primary Accession | Q8WTS6 |
Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Horse, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 41kDa |
Dilution | IHC-P (1:100), WB (1:500-1:1000), |
Gene ID | 80854 |
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Other Names | Histone-lysine N-methyltransferase SETD7, 2.1.1.43, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, SETD7, KIAA1717, KMT7, SET7, SET9 |
Target/Specificity | Two synthetic peptides corresponding to amino acids 131-145 (GEVNEDGEMTGEKIA) and336-352 (GYDHSPPGKSGPEAPEW) of human Set7/9 were used as immunogen; GenBank Accession No. NP_085151.1. Thesesequences are 100% conserved between human, mouse and rat. |
Reconstitution & Storage | Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
Precautions | SETD7 / SET7 Antibody (aa131-145) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SETD7 |
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Function | Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed:11779497, PubMed:11850410, PubMed:12588998, PubMed:12540855, PubMed:16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:12588998, PubMed:12540855, PubMed:16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed:16141209, PubMed:12588998). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed:16141209). Has also methyltransferase activity toward non- histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed:15099517, PubMed:35210392, PubMed:15525938, PubMed:16415881). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed:15099517, PubMed:16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed:17108971, PubMed:15525938, PubMed:16415881). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity). |
Cellular Location | Nucleus. Chromosome |
Tissue Location | Widely expressed. Expressed in pancreatic islets. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
References
Wang H.,et al.Mol. Cell 8:1207-1217(2001).
Nishioka K.,et al.Genes Dev. 16:479-489(2002).
Hillier L.W.,et al.Nature 434:724-731(2005).
Nagase T.,et al.DNA Res. 7:347-355(2000).
Martens J.H.,et al.Mol. Cell. Biol. 23:1808-1816(2003).
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