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NEDD8 Antibody (aa10-59)

Rabbit Polyclonal Antibody

     
  • WB - NEDD8 Antibody (aa10-59) ALS14631
    Western blot of extracts from Jurkat cells, using NEDD8 Antibody.
    detail
  • IHC - NEDD8 Antibody (aa10-59) ALS14631
    Anti-NEDD8 antibody IHC of human small intestine.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q15843
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 9kDa
Dilution ELISA (1:5000), IHC-P (1:100), WB (1:500-1:1000)
Additional Information
Gene ID 4738
Other Names NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8, Ubiquitin-like protein Nedd8, NEDD8
Target/Specificity NEDD8 Antibody detects endogenous levels of total NEDD8 protein.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsNEDD8 Antibody (aa10-59) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEDD8 {ECO:0000303|PubMed:9694792, ECO:0000312|HGNC:HGNC:7732}
Function Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:9694792, PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:15242646, PubMed:14690597). Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed:9694792, PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:20688984). Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed:15242646). Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed:14690597).
Cellular Location Nucleus. Note=Mainly nuclear.
Tissue Location Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes
Volume 50 µl
Research Areas
Citations (0)
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Background

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

References

Osaka F.,et al.Genes Dev. 12:2263-2268(1998).
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Liakopoulos D.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:5510-5515(1999).
Kamitani T.,et al.J. Biol. Chem. 272:28557-28562(1997).
Wada H.,et al.Biochem. Biophys. Res. Commun. 251:688-692(1998).

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Discontinued
Cat# ALS14631
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