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UBE1DC1 / UBA5 Antibody (aa281-330)

Rabbit Polyclonal Antibody

     
  • WB - UBE1DC1 / UBA5 Antibody (aa281-330) ALS14636
    Western blot of extracts from HepG2/293 cells, using UBA5 Antibody.
    detail
  • IHC - UBE1DC1 / UBA5 Antibody (aa281-330) ALS14636
    Anti-UBA5 antibody IHC of human kidney, tubules.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9GZZ9
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 45kDa
Dilution ELISA (1:5000), IHC-P (5 µg/ml), WB (1:500-1:1000)
Additional Information
Gene ID 79876
Other Names Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, UBA5, UBE1DC1
Target/Specificity UBA5 Antibody detects endogenous levels of total UBA5 protein.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsUBE1DC1 / UBA5 Antibody (aa281-330) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBA5 {ECO:0000303|PubMed:15071506, ECO:0000312|HGNC:HGNC:23230}
Function E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed:15071506, PubMed:18442052, PubMed:25219498, PubMed:20368332, PubMed:27653677, PubMed:26929408, PubMed:27545674, PubMed:30412706, PubMed:27545681). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1- E1 thioester and free AMP (PubMed:20368332, PubMed:27653677, PubMed:26929408, PubMed:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed:29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed:27653677). Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed:32160526). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity).
Cellular Location Cytoplasm. Nucleus Endoplasmic reticulum membrane. Golgi apparatus. Note=Localizes mainly in the cytoplasm, while it localizes to the nucleus in presence of SUMO2 (PubMed:18442052). Interaction with GABARAPL2 promotes localization to the endoplasmic reticulum membrane (PubMed:30990354)
Tissue Location Widely expressed..
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

E1-like enzyme which activates UFM1 and SUMO2.

References

Komatsu M.,et al.EMBO J. 23:1977-1986(2004).
Dou T.,et al.Mol. Biol. Rep. 32:265-271(2005).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).

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$ 467.50
Cat# ALS14636
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