UBE1DC1 / UBA5 Antibody (aa281-330)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q9GZZ9 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 45kDa |
Dilution | ELISA (1:5000), IHC-P (5 µg/ml), WB (1:500-1:1000) |
Gene ID | 79876 |
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Other Names | Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, UBA5, UBE1DC1 |
Target/Specificity | UBA5 Antibody detects endogenous levels of total UBA5 protein. |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | UBE1DC1 / UBA5 Antibody (aa281-330) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | UBA5 {ECO:0000303|PubMed:15071506, ECO:0000312|HGNC:HGNC:23230} |
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Function | E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed:15071506, PubMed:18442052, PubMed:25219498, PubMed:20368332, PubMed:27653677, PubMed:26929408, PubMed:27545674, PubMed:30412706, PubMed:27545681). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1- E1 thioester and free AMP (PubMed:20368332, PubMed:27653677, PubMed:26929408, PubMed:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed:29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed:27653677). Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed:32160526). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity). |
Cellular Location | Cytoplasm. Nucleus Endoplasmic reticulum membrane. Golgi apparatus. Note=Localizes mainly in the cytoplasm, while it localizes to the nucleus in presence of SUMO2 (PubMed:18442052). Interaction with GABARAPL2 promotes localization to the endoplasmic reticulum membrane (PubMed:30990354) |
Tissue Location | Widely expressed.. |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
E1-like enzyme which activates UFM1 and SUMO2.
References
Komatsu M.,et al.EMBO J. 23:1977-1986(2004).
Dou T.,et al.Mol. Biol. Rep. 32:265-271(2005).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
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