RNF144A / RNF144 Antibody (aa151-200)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | P50876 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 33kDa |
Dilution | ELISA (1:40000), IHC-P (1:100), WB (1:500-1:1000) |
Gene ID | 9781 |
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Other Names | E3 ubiquitin-protein ligase RNF144A, 6.3.2.-, RING finger protein 144A, UbcM4-interacting protein 4, Ubiquitin-conjugating enzyme 7-interacting protein 4, RNF144A, KIAA0161, RNF144, UBCE7IP4 |
Target/Specificity | RNF144A Antibody detects endogenous levels of total RNF144A protein. |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | RNF144A / RNF144 Antibody (aa151-200) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RNF144A |
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Synonyms | KIAA0161, RNF144, UBCE7IP4 |
Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:26216882). Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC during DNA damage (PubMed:24979766). Positively regulates DNA virus or exogenous cytosolic DNA-triggered innate immune response by mediating STING1 ubiquitination and increasing its 'Lys-6'-linked ubiquitination and translocation from the endoplasmic reticulum to the Golgi leading to downstream signaling pathways (PubMed:37955227). Plays a positive role in EGF-dependent cell proliferation by prolonging EGF/EGFR signaling during EGF stimulation through EGFR ubiquitination (PubMed:30171075). Increases ERK activity independently of EGFR signaling by promoting polyubiquitination and subsequent degradation of VRK3 in the cytosol (PubMed:33067254). |
Cellular Location | Cell membrane; Single-pass membrane protein. Cytoplasmic vesicle membrane. Endosome membrane. Endoplasmic reticulum membrane |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.
References
Nagase T.,et al.DNA Res. 3:17-24(1996).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Martinez-Noel G.,et al.FEBS Lett. 454:257-261(1999).
Ho S.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 111:E2646-E2655(2014).
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