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RNF144A / RNF144 Antibody (aa151-200)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P50876 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 33kDa |
| Dilution | ELISA (1:40000), IHC-P (1:100), WB (1:500-1:1000) |
| Gene ID | 9781 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RNF144A, 6.3.2.-, RING finger protein 144A, UbcM4-interacting protein 4, Ubiquitin-conjugating enzyme 7-interacting protein 4, RNF144A, KIAA0161, RNF144, UBCE7IP4 |
| Target/Specificity | RNF144A Antibody detects endogenous levels of total RNF144A protein. |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Precautions | RNF144A / RNF144 Antibody (aa151-200) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNF144A |
|---|---|
| Synonyms | KIAA0161, RNF144, UBCE7IP4 |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:26216882). Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC during DNA damage (PubMed:24979766). Positively regulates DNA virus or exogenous cytosolic DNA-triggered innate immune response by mediating STING1 ubiquitination and increasing its 'Lys-6'-linked ubiquitination and translocation from the endoplasmic reticulum to the Golgi leading to downstream signaling pathways (PubMed:37955227). Plays a positive role in EGF-dependent cell proliferation by prolonging EGF/EGFR signaling during EGF stimulation through EGFR ubiquitination (PubMed:30171075). Increases ERK activity independently of EGFR signaling by promoting polyubiquitination and subsequent degradation of VRK3 in the cytosol (PubMed:33067254). |
| Cellular Location | Cell membrane; Single-pass membrane protein. Cytoplasmic vesicle membrane. Endosome membrane. Endoplasmic reticulum membrane |
| Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.
References
Nagase T.,et al.DNA Res. 3:17-24(1996).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Martinez-Noel G.,et al.FEBS Lett. 454:257-261(1999).
Ho S.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 111:E2646-E2655(2014).
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