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ANGPT1 / Angiopoietin-1 Antibody (Internal)

Goat Polyclonal Antibody

     
  • IHC - ANGPT1 / Angiopoietin-1 Antibody (Internal) ALS14686
    Anti-ANGPT1 / Angiopoietin-1 antibody IHC of human lung, respiratory epithelium.
  • WB - ANGPT1 / Angiopoietin-1 Antibody (Internal) ALS14686
    HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag...
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q15389
Reactivity Human, Mouse, Rabbit, Monkey, Horse, Dog
Host Goat
Clonality Polyclonal
Calculated MW 58kDa
Dilution ELISA (1:32000), IHC-P (2.5 µg/ml), WB (1-2 µg/ml)
Additional Information
Gene ID 284
Other Names Angiopoietin-1, ANG-1, ANGPT1, KIAA0003
Target/Specificity Human ANGPT1 / Angiopoietin-1.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsANGPT1 / Angiopoietin-1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANGPT1
Synonyms KIAA0003
Function Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

References

Davis S.,et al.Cell 87:1161-1169(1996).
Nakatsukasa M.,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Shan Z.X.,et al.Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
Nomura N.,et al.DNA Res. 1:27-35(1994).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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$ 395.00
Cat# ALS14686
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