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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NPC / NPC1 Antibody (C-Terminus)   

NPC / NPC1 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • IF - NPC / NPC1 Antibody (C-Terminus) ALS14705
    Immunofluorescence of NPC1 in Mouse Kidney cells with NPC1 antibody at 20 ug/ml.
  • WB - NPC / NPC1 Antibody (C-Terminus) ALS14705
    Western blot of NPC1 in human kidney tissue lysate with NPC1 antibody at 1 ug/ml.
  • IHC - NPC / NPC1 Antibody (C-Terminus) ALS14705
    Anti-NPC1 antibody IHC of human brain, cortex.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession O15118
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 142kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 4864
Other Names Niemann-Pick C1 protein, NPC1
Target/Specificity Human NPC1
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsNPC / NPC1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPC1
Function Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.
Cellular Location Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein
Research Areas
Citations (0)

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Background

Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.

References

Carstea E.D.,et al.Science 277:228-231(1997).
Morris J.A.,et al.Biochem. Biophys. Res. Commun. 261:493-498(1999).
Bauer P.,et al.Hum. Mutat. 19:30-38(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 437:551-555(2005).

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$ 395.00
Cat# ALS14705
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