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>   home   >   Products   >   Primary Antibodies   >   POLR2A / RNA polymerase II Antibody (aa1585-1634)   

POLR2A / RNA polymerase II Antibody (aa1585-1634)

Rabbit Polyclonal Antibody

     
  • IF - POLR2A / RNA polymerase II Antibody (aa1585-1634) ALS14718
    Immunofluorescence of HeLa cells, using POLR2A antibody.
  • WB - POLR2A / RNA polymerase II Antibody (aa1585-1634) ALS14718
    Western blot of extracts from COS7 cells, treated with EGF 200 ng/ml 30', using POLR2A antibody.
  • IHC - POLR2A / RNA polymerase II Antibody (aa1585-1634) ALS14718
    Anti-POLR2A / RNA polymerase II antibody IHC of human testis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P24928
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 217kDa
Dilution ELISA (1:10000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) ,
Additional Information
Gene ID 5430
Other Names DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, 2.7.7.48, POLR2A, POLR2
Target/Specificity POLR2A antibody detects endogenous levels of total POLR2A protein.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsPOLR2A / RNA polymerase II Antibody (aa1585-1634) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLR2A
Synonyms POLR2
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA- dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Cellular Location Nucleus.
Volume 50 µl
Citations (0)

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Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA- dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

References

Wintzerith M.,et al.Nucleic Acids Res. 20:910-910(1992).
Mita K.,et al.Gene 159:285-286(1995).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Kershnar E.,et al.J. Biol. Chem. 273:34444-34453(1998).

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$ 395.00
Cat# ALS14718
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