FN1 / Fibronectin Antibody (clone 16E5)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IP |
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Primary Accession | P02751 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 16E5 |
Calculated MW | 263kDa |
Dilution | IHC-P (1:200), WB (1:2000), |
Gene ID | 2335 |
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Other Names | Fibronectin, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3, FN1, FN |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | FN1 / Fibronectin Antibody (clone 16E5) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FN1 (HGNC:3778) |
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Synonyms | FN |
Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). |
Cellular Location | Secreted, extracellular space, extracellular matrix. Secreted {ECO:0000250|UniProtKB:P11276} |
Tissue Location | Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level) (PubMed:29777959) Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine (PubMed:17614963). |
Volume | 50 µl |
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Background
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
References
Schor S.L.,et al.Breast Cancer Res. 3:373-379(2001).
Kay R.A.,et al.Cancer Res. 65:10742-10749(2005).
Kato S.,et al.DNA Res. 12:53-62(2005).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
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