|Application ||WB, IHC-P, E, IP|
|Dilution||IHC-P (1:400), WB (1:2000),|
|Other Names||Vimentin, VIM|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||Vimentin Antibody (clone 33C7) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.|
|Tissue Location||Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Ferrari S.,et al.Mol. Cell. Biol. 6:3614-3620(1986).
Honore B.,et al.Nucleic Acids Res. 18:6692-6692(1990).
Hartmann T.B.,et al.Br. J. Dermatol. 150:252-258(2004).
Zimbelmann R.,et al.Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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