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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Rho Kinase / ROCK1 Antibody (N-Terminus)   

Rho Kinase / ROCK1 Antibody (N-Terminus)

Rabbit Polyclonal Antibody

     
  • ICC - Rho Kinase / ROCK1 Antibody (N-Terminus) ALS14820
    Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/ml.
  • IF - Rho Kinase / ROCK1 Antibody (N-Terminus) ALS14820
    Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 ug/ml.
  • WB - Rho Kinase / ROCK1 Antibody (N-Terminus) ALS14820
    Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/ml in (A) the...
  • IHC - Rho Kinase / ROCK1 Antibody (N-Terminus) ALS14820
    Anti-ROCK1 antibody IHC of human brain, cortex.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession Q13464
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 158kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 6093
Other Names Rho-associated protein kinase 1, 2.7.11.1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK-I, p160 ROCK-1, p160ROCK, ROCK1
Target/Specificity Human ROCK1. ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsRho Kinase / ROCK1 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ROCK1
Function Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Note=Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
Tissue Location Detected in blood platelets.
Research Areas
Citations (0)

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Background

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

References

Ishizaki T.,et al.EMBO J. 15:1885-1893(1996).
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Van Eyk J.E.,et al.J. Biol. Chem. 273:23433-23439(1998).
Scanlan M.J.,et al.Int. J. Cancer 83:456-464(1999).

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$ 395.00
Cat# ALS14820
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