|Application ||WB, IHC-P|
|Dilution||IHC-P (5-10 µg/ml), WB (1-3 µg/ml),|
|Other Names||Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI, GLI1, GLI|
|Reconstitution & Storage||Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.|
|Precautions||GLI / GLI1 Antibody (aa940-990) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'- GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). May regulate the transcription of specific genes during normal development (PubMed:19706761). May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761). Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable).|
|Cellular Location||Cytoplasm. Nucleus. Note=Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441)|
|Tissue Location||Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761)|
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Provided below are standard protocols that you may find useful for product applications.
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
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Yoon J.W.,et al.Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
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Scherer S.E.,et al.Nature 440:346-351(2006).
Murone M.,et al.Nat. Cell Biol. 2:310-312(2000).
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