|Application ||WB, IHC-P, IF, E|
|Reactivity||Human, Mouse, Rat|
|Dilution||IF (35 µg/ml), IHC-P (10 µg/ml), WB (1:500-1:1000)|
|Other Names||BAG family molecular chaperone regulator 1, BAG-1, Bcl-2-associated athanogene 1, BAG1, HAP|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||BAG1 / BAG-1 Antibody (clone 2D3) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.|
|Cellular Location||Isoform 1: Nucleus. Cytoplasm. Note=Isoform 1 localizes predominantly to the nucleus Isoform 4: Cytoplasm. Nucleus. Note=Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria|
|Tissue Location||Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
Zeiner M.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:11465-11469(1995).
Takayama S.,et al.Genomics 35:494-498(1996).
Takayama S.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Wadle A.,et al.Int. J. Cancer 117:896-904(2005).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
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