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CD74 Antibody (clone PIN.1)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IP, Flo |
|---|---|
| Primary Accession | P04233 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | PIN.1 |
| Calculated MW | 34kDa |
| Dilution | IHC-P (10 µg/ml), WB (1 µg/ml), |
| Gene ID | 972 |
|---|---|
| Other Names | HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD74, CD74, DHLAG |
| Target/Specificity | Detects ~33-35 kD protein doublet, corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS-PAGE immunoblots. |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Precautions | CD74 Antibody (clone PIN.1) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CD74 (HGNC:1697) |
|---|---|
| Synonyms | DHLAG |
| Function | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. [Isoform p41]: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215). |
| Cellular Location | Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Secreted. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane |
| Tissue Location | Detected in urine (at protein level). [Isoform p33]: In B cells, represents 70% of total CD74 expression. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
References
Claesson L.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:7395-7399(1983).
Strubin M.,et al.EMBO J. 3:869-872(1984).
Kudo J.,et al.Nucleic Acids Res. 13:8827-8841(1985).
O'Sullivan D.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:4484-4488(1986).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
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