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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CRYAB / Alpha B Crystallin Antibody (clone 1A7.D5)   

CRYAB / Alpha B Crystallin Antibody (clone 1A7.D5)

Mouse Monoclonal Antibody

     
  • WB - CRYAB / Alpha B Crystallin Antibody (clone 1A7.D5) ALS14977
    Western blot analysis of AlphaB Crystallin in bovine cell lines using a 1:1000 dilution of CRYAB...
    detail
  • WB - CRYAB / Alpha B Crystallin Antibody (clone 1A7.D5) ALS14977
    Alpha B Crystallin (1A7. D5); AlphaA (L); AlphaB (R).
    detail
  • IHC - CRYAB / Alpha B Crystallin Antibody (clone 1A7.D5) ALS14977
    Anti-CRYAB / Alpha B Crystallin antibody IHC of human kidney, tubules.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P02511
Reactivity Human, Bovine
Host Mouse
Clonality Monoclonal
Clone Names 1A7.D5
Calculated MW 20kDa
Dilution IHC-P (20 µg/ml), WB (1:2000),
Additional Information
Gene ID 1410
Other Names Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, CRYAB, CRYA2
Target/Specificity Detects a band at ~20kD (Predicted mol. weight is ~21 kD) corresponding to alpha B crystallin. Does not cross react with alpha A crystallin, beta-L crystallin, Beta-H crystallin, gamma crystallin, Hsp25, Hsp27 or Hsp47 proteins.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsCRYAB / Alpha B Crystallin Antibody (clone 1A7.D5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CRYAB (HGNC:2389)
Synonyms CRYA2, HSPB5
Function May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).
Cellular Location Cytoplasm. Nucleus Secreted. Lysosome {ECO:0000250|UniProtKB:P23927}. Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (PubMed:19464326). Localizes at the Z- bands and the intercalated disk in cardiomyocytes (PubMed:28493373) Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum- Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059).
Tissue Location Lens as well as other tissues (PubMed:838078, PubMed:2387586). Expressed in myocardial tissue (PubMed:28493373)
Research Areas
Citations (0)
citation

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Background

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.

References

Kramps J.A.,et al.FEBS Lett. 74:82-84(1977).
Dubin R.A.,et al.Genomics 7:594-601(1990).
Iwaki A.,et al.Neurosci. Lett. 140:89-92(1992).
Yu W.,et al.Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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Discontinued
Cat# ALS14977
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