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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HSPB1 / HSP27 Antibody (clone 5D12-A12)   

HSPB1 / HSP27 Antibody (clone 5D12-A12)

Mouse Monoclonal Antibody

  • WB - HSPB1 / HSP27 Antibody (clone 5D12-A12) ALS14978
    Hsp27(5D12-A3), HeLa lines.
  • IHC - HSPB1 / HSP27 Antibody (clone 5D12-A12) ALS14978
    Anti-HSPB1 antibody IHC of human heart.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04792
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5D12-A12
Calculated MW 23kDa
Dilution IHC-P (10 µg/ml), WB (0.25-0.5 µg/ml),
Additional Information
Gene ID 3315
Other Names Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28
Target/Specificity Detects a 27kD protein corresponding to the molecular mass of hsp27 on SDS PAGE immunoblots. There is no cross-reactivity to alphaBcrystallin.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsHSPB1 / HSP27 Antibody (clone 5D12-A12) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPB1
Synonyms HSP27, HSP28
Function Involved in stress resistance and actin organization.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
Tissue Location Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle
Research Areas
Citations (0)

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Involved in stress resistance and actin organization.


Hickey E.,et al.Nucleic Acids Res. 14:4127-4145(1986).
Carper S.W.,et al.Nucleic Acids Res. 18:6457-6457(1990).
Hino M.,et al.Biochem. Biophys. Res. Commun. 271:164-169(2000).
Briolay J.,et al.Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
Yu W.,et al.Genome Res. 7:353-358(1997).

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$ 395.00
Cat# ALS14978
(40 western blots)
Availability: 5 days
Bulk Size
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