|Application ||WB, IHC-P, E, IP|
|Dilution||IHC-P (10 µg/ml), WB (0.25-0.5 µg/ml),|
|Other Names||Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28|
|Target/Specificity||Detects a 27kD protein corresponding to the molecular mass of hsp27 on SDS PAGE immunoblots. There is no cross-reactivity to alphaBcrystallin.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||HSPB1 / HSP27 Antibody (clone 5D12-A12) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in stress resistance and actin organization.|
|Cellular Location||Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles|
|Tissue Location||Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle|
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Provided below are standard protocols that you may find useful for product applications.
Involved in stress resistance and actin organization.
Hickey E.,et al.Nucleic Acids Res. 14:4127-4145(1986).
Carper S.W.,et al.Nucleic Acids Res. 18:6457-6457(1990).
Hino M.,et al.Biochem. Biophys. Res. Commun. 271:164-169(2000).
Briolay J.,et al.Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
Yu W.,et al.Genome Res. 7:353-358(1997).
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