|Application ||WB, IHC-P, E|
|Dilution||IHC-P (10 µg/ml), WB (1:2000),|
|Other Names||Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, CRYAB, CRYA2|
|Target/Specificity||Detects a band at ~20kD (Predicted mol. weight is ~21 kD) corresponding to alpha B crystallin. Does not cross react with alpha A crystallin, beta-L crystallin, Beta-H crystallin, gamma crystallin, Hsp25, Hsp27 or Hsp47 proteins.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||CRYAB / Alpha B Crystallin Antibody (clone 3A10.H4) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles|
|Tissue Location||Lens as well as other tissues.|
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Provided below are standard protocols that you may find useful for product applications.
May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
Kramps J.A.,et al.FEBS Lett. 74:82-84(1977).
Dubin R.A.,et al.Genomics 7:594-601(1990).
Iwaki A.,et al.Neurosci. Lett. 140:89-92(1992).
Yu W.,et al.Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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