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>   home   >   Products   >   Primary Antibodies   >   EEF2 / Elongation Factor 2 Antibody (aa31-80)   

EEF2 / Elongation Factor 2 Antibody (aa31-80)

Rabbit Polyclonal Antibody

     
  • IF - EEF2 / Elongation Factor 2 Antibody (aa31-80) ALS15007
    Immunofluorescence of HUVEC cells, using eEF2 Antibody.
    detail
  • WB - EEF2 / Elongation Factor 2 Antibody (aa31-80) ALS15007
    Western blot of extracts from NIH-3T3 cells, treated with serum 10% 30', using eEF2 Antibody.
    detail
  • IHC - EEF2 / Elongation Factor 2 Antibody (aa31-80) ALS15007
    Anti-EEF2 antibody IHC of human prostate.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P13639
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 95kDa
Dilution ELISA (1:5000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) ,
Additional Information
Gene ID 1938
Other Names Elongation factor 2, EF-2, EEF2, EF2
Target/Specificity eEF2 Antibody detects endogenous levels of total eEF2 protein.
Reconstitution & Storage Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PrecautionsEEF2 / Elongation Factor 2 Antibody (aa31-80) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF2
Synonyms EF2
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl- tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed:26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed:26593721).
Cellular Location Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C- terminal cleavage product.
Volume 50 µl
Citations (0)
citation

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Background

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

References

Rapp G.,et al.Biol. Chem. Hoppe-Seyler 370:1071-1075(1989).
Hanes J.,et al.Biol. Chem. Hoppe-Seyler 373:201-204(1992).
Ustek D.,et al.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Rapp G.,et al.Biol. Chem. Hoppe-Seyler 369:247-250(1988).

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Discontinued
Cat# ALS15007
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