|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:5000), IHC-P (10 µg/ml), WB (1:500-1:1000)|
|Other Names||SUMO-activating enzyme subunit 2, 6.3.2.-, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2, UBA2, SAE2, UBLE1B|
|Target/Specificity||Uba2 Antibody detects endogenous levels of total Uba2 protein.|
|Reconstitution & Storage||Store at -20°C for up to one year.|
|Precautions||SAE2 / UBA2 Antibody (aa591-640) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention|
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Provided below are standard protocols that you may find useful for product applications.
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Okuma T.,et al.Biochem. Biophys. Res. Commun. 254:693-698(1999).
Gong L.,et al.FEBS Lett. 448:185-189(1999).
Desterro J.M.P.,et al.J. Biol. Chem. 274:10618-10624(1999).
Slapak C.,et al.Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).
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