|Application ||WB, IHC-P, IF, E|
|Dilution||ELISA (1:20000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) ,|
|Other Names||Serine/threonine-protein kinase B-raf, 22.214.171.124, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1|
|Target/Specificity||B-RAF Antibody detects endogenous levels of total B-RAF protein.|
|Reconstitution & Storage||Store at -20°C for up to one year.|
|Precautions||BRAF / B-Raf Antibody (aa171-220) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.|
|Cellular Location||Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.|
|Tissue Location||Brain and testis.|
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Provided below are standard protocols that you may find useful for product applications.
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Stephens R.M.,et al.Mol. Cell. Biol. 12:3733-3742(1992).
Albert S.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W.,et al.Science 300:767-772(2003).
Hillier L.W.,et al.Nature 424:157-164(2003).
Eychene A.,et al.Oncogene 7:1657-1660(1992).
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