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DACT1 / DAPPER Antibody (aa725-775)

Rabbit Polyclonal Antibody

     
  • WB - DACT1 / DAPPER Antibody (aa725-775) ALS15241
    Western blot of Dapper homolog 1inbrain lysate.
  • IHC - DACT1 / DAPPER Antibody (aa725-775) ALS15241
    Anti-DACT1 / DAPPER antibody IHC of human brain, cortex neurons.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q9NYF0
Reactivity Human, Mouse, Rat, Zebrafish, Chicken, Xenopus
Host Rabbit
Clonality Polyclonal
Calculated MW 90kDa
Dilution IHC-P (10 µg/ml), WB (0.5-1 µg/ml),
Additional Information
Gene ID 51339
Other Names Dapper homolog 1, hDPR1, Dapper antagonist of catenin 1, Hepatocellular carcinoma novel gene 3 protein, DACT1, DPR1, HNG3
Target/Specificity Human DACT1
Reconstitution & Storage Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.
PrecautionsDACT1 / DAPPER Antibody (aa725-775) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DACT1
Synonyms DPR1, HNG3
Function Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome- dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway (By similarity). The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.
Cellular Location Cytoplasm. Nucleus. Cell junction, synapse. Note=Shuttles between the nucleus and the cytoplasm. Seems to be nuclear in the absence of Wnt signaling and to translocate to the cytoplasm in its presence
Research Areas
Citations (0)

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Background

Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome- dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway (By similarity). The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.

References

Heilig R.,et al.Nature 421:601-607(2003).
Li W.B.,et al.Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
Gong S.,et al.Zhongguo Sheng Wu Hua Xue Yu Fen Zi Sheng Wu Xue Bao 17:280-287(2001).
Cheyette B.N.R.,et al.Dev. Cell 2:449-461(2002).
Yau T.O.,et al.Oncogene 24:1607-1614(2005).

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$ 395.00
Cat# ALS15241
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