|Application ||WB, IHC-P|
|Dilution||IHC-P (10 µg/ml), WB (0.25-0.5 µg/ml),|
|Other Names||Switch-associated protein 70, SWAP-70, SWAP70, KIAA0640|
|Reconstitution & Storage||Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.|
|Precautions||SWAP70 Antibody (aa1-50) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).|
|Cellular Location||Cytoplasm. Cell membrane. Nucleus. Cell projection, lamellipodium Cytoplasm, cytoskeleton. Note=In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus (PubMed:10681448). In activated, class-switching B-cells it is associated with membrane IgG but not IgM (PubMed:10681448). Localized to loose actin filament arrays located behind actively extending lamellipodia (PubMed:12925760)|
|Tissue Location||Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus- associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760)|
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Provided below are standard protocols that you may find useful for product applications.
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).
Masat L.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:2180-2184(2000).
Rapalus L.,et al.Clin. Immunol. 101:270-275(2001).
Monz D.W.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Ishikawa K.,et al.DNA Res. 5:169-176(1998).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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