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EIF3A Antibody (aa550-600)

Rabbit Polyclonal Antibody

     
  • WB - EIF3A Antibody (aa550-600) ALS15253
    Western blot of eIF3A in Daudi cell lysate in the 1) absence and 2) presence of immunizing...
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q14152
Reactivity Human, Mouse, Rat, Chicken, Horse, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 167kDa
Dilution IHC-P (10 µg/ml), WB (0.25-1 µg/ml),
Additional Information
Gene ID 8661
Other Names Eukaryotic translation initiation factor 3 subunit A {ECO:0000255|HAMAP-Rule:MF_03000}, eIF3a {ECO:0000255|HAMAP-Rule:MF_03000}, Eukaryotic translation initiation factor 3 subunit 10 {ECO:0000255|HAMAP-Rule:MF_03000}, eIF-3-theta {ECO:0000255|HAMAP-Rule:MF_03000}, eIF3 p167, eIF3 p180, eIF3 p185, EIF3A {ECO:0000255|HAMAP-Rule:MF_03000}
Target/Specificity Human EIF3A
Reconstitution & Storage Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
PrecautionsEIF3A Antibody (aa550-600) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF3A {ECO:0000255|HAMAP-Rule:MF_03000}
Function RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl- tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem- loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03000, ECO:0000269|PubMed:9150439}
Citations (0)
citation

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Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

References

Scholler J.K.,et al.DNA Cell Biol. 16:515-531(1997).
Johnson K.R.,et al.J. Biol. Chem. 272:7106-7113(1997).
Nagase T.,et al.DNA Res. 2:167-174(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).

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Discontinued
Cat# ALS15253
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