|Application ||WB, IHC-P, IF, ICC|
|Reactivity||Human, Mouse, Rat|
|Dilution||IHC-P (5 µg/ml)|
|Other Names||Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, 188.8.131.52, Endoribonuclease, 3.1.26.-, ERN1 (HGNC:3449)|
|Target/Specificity||16 amino acid peptide from near the carboxy terminus of human IRE1p|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||ERN1 / IRE1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type I membrane protein|
|Tissue Location||Ubiquitously expressed. High levels observed in pancreatic tissue.|
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Provided below are standard protocols that you may find useful for product applications.
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Tirasophon W.,et al.Genes Dev. 12:1812-1824(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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