|Application ||WB, IHC-P, IF, ICC, E, IP, GS|
|Reactivity||Human, Mouse, Rat, Rabbit, Hamster, Monkey, Bovine, Guinea Pig|
|Dilution||IHC-P (10 µg/ml)|
|Other Names||Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1|
|Target/Specificity||Detects an ~85kDa protein inunstressed cell lysates, and an~95kDa protein in heat shockedcell lysates, corresponding tothe molecular mass of inactiveand active forms of HSF1 onSDS-PAGE immunoblots.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||HSF1 Antibody (clone 10H8) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.|
|Cellular Location||Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Rabindran S.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Nusbaum C.,et al.Nature 439:331-335(2006).
Schuetz T.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.