|Application ||WB, IHC-P, IF, ICC, E, IP, GS|
|Reactivity||Human, Mouse, Rat, Rabbit, Hamster, Monkey, Bovine, Guinea Pig|
|Dilution||IHC-P (10 µg/ml)|
|Other Names||Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1|
|Target/Specificity||Detects an ~85kDa protein inunstressed cell lysates, and an~95kDa protein in heat shockedcell lysates, corresponding tothe molecular mass of inactiveand active forms of HSF1 onSDS-PAGE immunoblots.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||HSF1 Antibody (clone 10H8) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.|
|Cellular Location||Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules|
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Provided below are standard protocols that you may find useful for product applications.
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Rabindran S.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Nusbaum C.,et al.Nature 439:331-335(2006).
Schuetz T.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991).
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