|Application ||WB, IHC-P, IP|
|Reactivity||Human, Mouse, Rat|
|Dilution||IHC-P (10 µg/ml)|
|Other Names||Copper-transporting ATPase 1, 220.127.116.11, Copper pump 1, Menkes disease-associated protein, ATP7A, MC1, MNK|
|Target/Specificity||Detects ~180kDa in rat brainmembrane preparations.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||MNK / ATP7A Antibody (clone S60-4) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Cycles constitutively between the trans-Golgi network (TGN) and the plasma membrane (PubMed:9147644) Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels Isoform 5: Endoplasmic reticulum|
|Tissue Location||Found in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.
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Vulpe C.D.,et al.Nat. Genet. 3:273-273(1993).
Tuemer Z.,et al.Genomics 26:437-442(1995).
Reddy M.C.,et al.Biochem. J. 334:71-77(1998).
Harris E.D.,et al.Adv. Exp. Med. Biol. 448:39-51(1999).
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