|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat, Rabbit, Hamster, Monkey, Bovine, Dog|
|Dilution||ELISA (1:64000), IHC-P (3.75 µg/ml), WB (0.3-1 µg/ml)|
|Other Names||Prostatic acid phosphatase, PAP, 188.8.131.52, 5'-nucleotidase, 5'-NT, 184.108.40.206, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase, PAPf39, ACPP|
|Target/Specificity||Human ACPP. This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||ACPP / PAP Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.|
|Cellular Location||Isoform 1: Secreted|
|Tissue Location||Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Isoform 2 also expressed in the sarcolemma of skeletal muscle. Levels of this cellular isoform decreased in prostate cancer.|
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Provided below are standard protocols that you may find useful for product applications.
A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
Sharief F.S.,et al.Biochem. Biophys. Res. Commun. 184:1468-1476(1992).
van Etten R.L.,et al.J. Biol. Chem. 266:2313-2319(1991).
Sharief F.S.,et al.Biochem. Biophys. Res. Commun. 160:79-86(1989).
Vihko P.,et al.FEBS Lett. 236:275-281(1988).
Tailor P.G.,et al.Nucleic Acids Res. 18:4928-4928(1990).
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