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MCM3AP / GANP Antibody (aa1841-1890)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O60318 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 218kDa |
| Dilution | ELISA (1:5000), IHC-P (5 µg/ml), WB (1:500-1:1000) |
| Gene ID | 8888 |
|---|---|
| Other Names | Germinal-center associated nuclear protein, GANP, 80 kDa MCM3-associated protein, MCM3 acetylating protein, MCM3AP, 2.3.1.-, MCM3 acetyltransferase, MCM3AP, GANP, KIAA0572, MAP80 |
| Target/Specificity | GANP Antibody detects endogenous levels of total GANP protein. |
| Reconstitution & Storage | Store at -20°C for up to one year. |
| Precautions | MCM3AP / GANP Antibody (aa1841-1890) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MCM3AP |
|---|---|
| Synonyms | GANP, KIAA0572, MAP80 |
| Function | [Isoform GANP]: As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (PubMed:20005110, PubMed:20384790, PubMed:23591820, PubMed:22307388). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed:23652018). |
| Cellular Location | [Isoform GANP]: Nucleus envelope. Nucleus, nuclear pore complex. Nucleus, nucleoplasm. Chromosome. Note=Predominantly located at the nuclear envelope, facing the nucleus interior (PubMed:20005110, PubMed:21195085, PubMed:23591820). Localization at the nuclear pore complex requires NUP153, TPR and ALYREF/ALY (PubMed:23591820, PubMed:22307388). Also found associated with chromatin (PubMed:23652018). In B-cells, targeted to the immunoglobulin variable region genes (PubMed:23652018). |
| Tissue Location | Widely expressed (PubMed:11024281). Up-regulated in germinal center B-cells in tonsils (at protein level) (PubMed:11024281). |
| Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Isoform GANP: Essential for the generation of high- affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation- dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells (By similarity). Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.
References
Abe E.,et al.Gene 255:219-227(2000).
Hattori M.,et al.Nature 405:311-319(2000).
Nagase T.,et al.DNA Res. 5:31-39(1998).
Takei Y.,et al.J. Biol. Chem. 273:22177-22180(1998).
Takei Y.,et al.EMBO Rep. 2:119-123(2001).
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