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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   AKAP8 / AKAP95 Antibody (aa331-380)   

AKAP8 / AKAP95 Antibody (aa331-380)

Rabbit Polyclonal Antibody

     
  • IF - AKAP8 / AKAP95 Antibody (aa331-380) ALS15432
    Immunofluorescence of HUVEC cells, using AKAP8 Antibody.
  • WB - AKAP8 / AKAP95 Antibody (aa331-380) ALS15432
    Western blot of extracts from mouse heart/Jurkat cells, using AKAP8 Antibody.
  • IHC - AKAP8 / AKAP95 Antibody (aa331-380) ALS15432
    Anti-AKAP8 / AKAP95 antibody IHC of human colon.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession O43823
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 76kDa
Dilution ELISA (1:20000), IF (1:100-1:500), IHC-P (5 µg/ml), WB (1:500-1:1000) ,
Additional Information
Gene ID 10270
Other Names A-kinase anchor protein 8, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95, AKAP8, AKAP95
Target/Specificity AKAP8 Antibody detects endogenous levels of total AKAP8 protein.
Reconstitution & Storage Store at -20°C for up to one year.
PrecautionsAKAP8 / AKAP95 Antibody (aa331-380) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKAP8
Synonyms AKAP95
Function Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity).
Cellular Location Nucleus. Nucleus matrix. Nucleus, nucleolus. Cytoplasm {ECO:0000250|UniProtKB:Q9DBR0}. Note=Associated with the nuclear matrix in interphase and redistributes mostly to chromatin at mitosis. However, mitotic chromatin localization has been questioned. Upon nuclear reassembly at the end of mitosis, is sequestered into the daughter nuclei where it re-acquires an interphase distribution. Localized to the nucleolus in interphase Colocalizes with GJA1 at the nuclear membrane specifically during cell cycle G1/S phase.
Tissue Location Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells
Volume 50 µl
Research Areas
Citations (0)

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Background

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).

References

Eide T.,et al.Exp. Cell Res. 238:305-316(1998).
Grimwood J.,et al.Nature 428:529-535(2004).
Olsen J.V.,et al.Cell 127:635-648(2006).
Daub H.,et al.Mol. Cell 31:438-448(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

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$ 395.00
Cat# ALS15432
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