|Application ||WB, IHC-P|
|Dilution||IHC-P (10 µg/ml)|
|Other Names||IST1 homolog, hIST1, Putative MAPK-activating protein PM28, IST1, KIAA0174|
|Reconstitution & Storage||Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.|
|Precautions||IST1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells (PubMed:19129479, PubMed:19129480). During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing (PubMed:26040712). Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST (PubMed:23897888).|
|Cellular Location||Cytoplasmic vesicle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody. Nucleus envelope. Note=Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964). Colocalized with SPG20 to the ends of Flemming bodies during cytokinesis (PubMed:20719964). Localizes to the nuclear envelope during late anaphase (PubMed:26040712)|
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Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
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Matsuda A.,et al.Oncogene 22:3307-3318(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Martin J.,et al.Nature 432:988-994(2004).
Rush J.,et al.Nat. Biotechnol. 23:94-101(2005).
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