|Application ||WB, IHC-P|
|Dilution||IHC-P (10 µg/ml)|
|Other Names||IST1 homolog, hIST1, Putative MAPK-activating protein PM28, IST1, KIAA0174|
|Reconstitution & Storage||Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.|
|Precautions||IST1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells (PubMed:19129479, PubMed:19129480). During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing (PubMed:26040712). Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST (PubMed:23897888).|
|Cellular Location||Cytoplasmic vesicle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody. Nucleus envelope. Note=Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964). Colocalized with SPG20 to the ends of Flemming bodies during cytokinesis (PubMed:20719964). Localizes to the nuclear envelope during late anaphase (PubMed:26040712)|
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Provided below are standard protocols that you may find useful for product applications.
Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
Nagase T.,et al.DNA Res. 3:17-24(1996).
Matsuda A.,et al.Oncogene 22:3307-3318(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Martin J.,et al.Nature 432:988-994(2004).
Rush J.,et al.Nat. Biotechnol. 23:94-101(2005).
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