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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EPM2A / Laforin Antibody (C-Terminus)   

EPM2A / Laforin Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • IHC - EPM2A / Laforin Antibody (C-Terminus) ALS15656
    Anti-EPM2A / Laforin antibody IHC staining of human small intestine.
  • ICC - EPM2A / Laforin Antibody (C-Terminus) ALS15656
    Immunocytochemistry of EPM2A in SW480 cells with EPM2A antibody at 2.5 ug/ml.
  • IF - EPM2A / Laforin Antibody (C-Terminus) ALS15656
    Immunofluorescence of EPM2A in SW480 cells with EPM2A antibody at 5 ug/ml.
  • WB - EPM2A / Laforin Antibody (C-Terminus) ALS15656
    Western blot analysis of SW480 in SW480 cell lysate with EPM2A antibody at (A) 1 and (B) 2 ug/ml.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession O95278
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 37kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 7957
Other Names Laforin, 3.1.3.-, 3.1.3.16, 3.1.3.48, Glucan phosphatase, Lafora PTPase, LAFPTPase, EPM2A
Target/Specificity Human EPM2A. At least four isoforms of EPM2A are known to exist; this antibody will detect all but the shortest isoform.
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsEPM2A / Laforin Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPM2A
Function Has both dual-specificity protein phosphatase and glucan phosphatase activities. Together with the E3 ubiquitin ligase NHLRC1/malin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. Dephosphorylates phosphotyrosine, phosphoserine and phosphothreonine substrates in vitro. Has also been shown to dephosphorylate MAPT. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates. Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway. Isoform 2, an inactive phosphatase, could function as a dominant-negative regulator for the phosphatase activity of isoform 1.
Cellular Location Cytoplasm Note=Under glycogenolytic conditions localizes to the nucleus Isoform 2: Endoplasmic reticulum. Cell membrane. Nucleus. Note=Also found in the nucleus Isoform 5: Cytoplasm. Nucleus.
Tissue Location Expressed in heart, skeletal muscle, kidney, pancreas and brain. Isoform 4 is also expressed in the placenta
Research Areas
Citations (0)

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Background

Has both dual-specificity protein phosphatase and glucan phosphatase activities. Together with the E3 ubiquitin ligase NHLRC1/malin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. Dephosphorylates phosphotyrosine, phosphoserine and phosphothreonine substrates in vitro. Has also been shown to dephosphorylate MAPT. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates. Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway. Isoform 2, an inactive phosphatase, could function as a dominant-negative regulator for the phosphatase activity of isoform 1.

References

Minassian B.A.,et al.Nat. Genet. 20:171-174(1998).
Ganesh S.,et al.Hum. Mol. Genet. 9:2251-2261(2000).
Lee J.R.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Ganesh S.,et al.Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS15656
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