|Application ||WB, IHC-P, IF|
|Dilution||IHC-P (2.5 µg/ml), WB (1-2 µg/ml),|
|Other Names||Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, 188.8.131.52, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, IRAP, Oxytocinase, OTase, Placental leucine aminopeptidase, P-LAP, Leucyl-cystinyl aminopeptidase, pregnancy serum form, LNPEP, OTASE|
|Target/Specificity||Human LNPEP. At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||LNPEP Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Note=In brain only the membrane- bound form is found. The protein resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Localization may be determined by dileucine internalization motifs, and/or by interaction with tankyrases|
|Tissue Location||Highly expressed in placenta, heart, kidney and small intestine. Detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon|
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Provided below are standard protocols that you may find useful for product applications.
Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.
Rogi T.,et al.J. Biol. Chem. 271:56-61(1996).
Laustsen P.G.,et al.Biochim. Biophys. Acta 1352:1-7(1997).
Rasmussen T.E.,et al.Eur. J. Biochem. 267:2297-2306(2000).
Matsumoto H.,et al.Eur. J. Biochem. 268:3259-3266(2001).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
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