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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Mitochondrion   >   GK / Glycerol Kinase Antibody (aa461-510)   

GK / Glycerol Kinase Antibody (aa461-510)

Rabbit Polyclonal Antibody

     
  • IHC - GK / Glycerol Kinase Antibody (aa461-510) ALS15757
    Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
    detail
  • IF - GK / Glycerol Kinase Antibody (aa461-510) ALS15757
    Immunofluorescence of HeLa cells, using GK Antibody.
    detail
  • WB - GK / Glycerol Kinase Antibody (aa461-510) ALS15757
    Western blot of extracts from 293/Jurkat cells, using GK Antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P32189
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 61kDa
Dilution ELISA (1:20000), IF (1:100-1:500), IHC-P (20 µg/ml), WB (1:500-1:1000) ,
Additional Information
Gene ID 2710
Other Names Glycerol kinase, GK, Glycerokinase, 2.7.1.30, ATP:glycerol 3-phosphotransferase, GK
Target/Specificity GK Antibody detects endogenous levels of total GK protein.
Reconstitution & Storage Store at -20°C for up to one year.
PrecautionsGK / Glycerol Kinase Antibody (aa461-510) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GK (HGNC:4289)
Function Kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3-phosphate. Sn- glycerol 3-phosphate is a crucial intermediate in various metabolic pathways, such as the synthesis of glycerolipids and triglycerides, glycogenesis, glycolysis and gluconeogenesis.
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein. Nucleus. Cytoplasm, cytosol. Note=Glycerol kinase activity is more cytosolic in some tissues. It probably represents the expression of isoforms lacking a transmembrane domain [Isoform 4]: Cytoplasm, cytosol. Note=In adult tissues, such as liver the glycerol kinase activity is more cytosolic. It probably represents the expression of this isoform which lacks a transmembrane domain
Tissue Location [Isoform 2]: Widely expressed in fetal and adult tissues. [Isoform 4]: The sole isoform expressed in adult liver and kidney.
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

Key enzyme in the regulation of glycerol uptake and metabolism.

References

Guo W.,et al.Nat. Genet. 4:367-372(1993).
Sargent C.A.,et al.Hum. Mol. Genet. 3:1317-1324(1994).
Sargent C.A.,et al.J. Med. Genet. 37:434-441(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ross M.T.,et al.Nature 434:325-337(2005).

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Discontinued
Cat# ALS15757
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