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BHLHE41 / BHLHB3 / SHARP1 Antibody (aa1-50)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9C0J9 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 50kDa |
| Dilution | ELISA (1:40000), IHC-P (20 µg/ml), WB (1:500-1:1000) |
| Gene ID | 79365 |
|---|---|
| Other Names | Class E basic helix-loop-helix protein 41, bHLHe41, Class B basic helix-loop-helix protein 3, bHLHb3, Differentially expressed in chondrocytes protein 2, hDEC2, Enhancer-of-split and hairy-related protein 1, SHARP-1, BHLHE41, BHLHB3, DEC2, SHARP1 |
| Target/Specificity | BHLHB3 Antibody detects endogenous levels of total BHLHB3 protein. |
| Reconstitution & Storage | Store at -20°C for up to one year. |
| Precautions | BHLHE41 / BHLHB3 / SHARP1 Antibody (aa1-50) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BHLHE41 (HGNC:16617) |
|---|---|
| Function | Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed:11278948, PubMed:14672706, PubMed:15193144, PubMed:15560782, PubMed:18411297, PubMed:19786558, PubMed:25083013). Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed:25083013). Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity). |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981} |
| Tissue Location | Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.
References
Fujimoto K.,et al.Biochem. Biophys. Res. Commun. 280:164-171(2001).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Garriga-Canut M.,et al.J. Biol. Chem. 276:14821-14828(2001).
Kawamoto T.,et al.Biochem. Biophys. Res. Commun. 313:117-124(2004).
Li Y.,et al.Biochem. J. 382:895-904(2004).
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