|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:32000), IHC-P (3.75 µg/ml), WB (0.01-0.03 µg/ml)|
|Other Names||NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, 22.214.171.124, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, CYB5R3, DIA1|
|Target/Specificity||Human CYB5R3. This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||CYB5R3 / B5R Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.|
|Cellular Location||Isoform 1: Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Mitochondrion outer membrane; Lipid-anchor; Cytoplasmic side|
|Tissue Location||Isoform 2 is expressed at late stages of erythroid maturation|
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Provided below are standard protocols that you may find useful for product applications.
Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Tomatsu S.,et al.Gene 80:353-361(1989).
Voice M.W.,et al.Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
Yoon B.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
Lan F.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
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