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PSAP / Prosaposin Antibody (clone 4D5F4)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC, E, Flo |
|---|---|
| Primary Accession | P07602 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4D5F4 |
| Calculated MW | 58kDa |
| Dilution | ELISA (1:10000), Flo (1:200-1:400), ICC (1:50-1:500), IF (1:500), IHC-P (5 µg/ml), WB (1:500-1:2000), |
| Gene ID | 5660 |
|---|---|
| Other Names | Prosaposin, Proactivator polypeptide, Saposin-A, Protein A, Saposin-B-Val, Saposin-B, Cerebroside sulfate activator, CSAct, Dispersin, Sphingolipid activator protein 1, SAP-1, Sulfatide/GM1 activator, Saposin-C, A1 activator, Co-beta-glucosidase, Glucosylceramidase activator, Sphingolipid activator protein 2, SAP-2, Saposin-D, Component C, Protein C, PSAP, GLBA, SAP1 |
| Target/Specificity | Human Prosaposin |
| Reconstitution & Storage | Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
| Precautions | PSAP / Prosaposin Antibody (clone 4D5F4) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PSAP |
|---|---|
| Synonyms | GLBA, SAP1 |
| Function | Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin- C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12). Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases. |
| Cellular Location | Lysosome |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12). Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
References
Rorman E.G.,et al.Genomics 5:486-492(1989).
Nakano T.,et al.J. Biochem. 105:152-154(1989).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Deloukas P.,et al.Nature 429:375-381(2004).
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