|Application ||WB, IHC-P, IF|
|Dilution||IF (1:100), IHC-P (10 µg/ml), WB (1:4000)|
|Other Names||SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SUMO-activating enzyme subunit 1, N-terminally processed, SAE1, AOS1, SUA1, UBLE1A|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||SAE1 Antibody (clone 1G8) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AOS1, SUA1, UBLE1A|
|Function||The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.|
|Tissue Location||Expression level increases during S phase and drops in G2 phase (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Okuma T.,et al.Biochem. Biophys. Res. Commun. 254:693-698(1999).
Gong L.,et al.FEBS Lett. 448:185-189(1999).
Desterro J.M.P.,et al.J. Biol. Chem. 274:10618-10624(1999).
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Li W.B.,et al.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
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