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PGAM2 Antibody (clone 4E9)

Mouse Monoclonal Antibody

     
  • WB - PGAM2 Antibody (clone 4E9) ALS15839
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2...
    detail
  • WB - PGAM2 Antibody (clone 4E9) ALS15839
    Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal...
    detail
  • IF - PGAM2 Antibody (clone 4E9) ALS15839
    Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently...
    detail
  • IF - PGAM2 Antibody (clone 4E9) ALS15839
    Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.
    detail
  • IHC - PGAM2 Antibody (clone 4E9) ALS15839
    Anti-PGAM2 antibody IHC staining of human skeletal muscle.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC
Primary Accession P15259
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 4E9
Calculated MW 29kDa
Dilution Flo (1:100), IF (1:50-1:100), IHC-P (1:150), WB (1:500-1:2000) ,
Additional Information
Gene ID 5224
Other Names Phosphoglycerate mutase 2, 3.1.3.13, 5.4.2.11, 5.4.2.4, BPG-dependent PGAM 2, Muscle-specific phosphoglycerate mutase, Phosphoglycerate mutase isozyme M, PGAM-M, PGAM2, PGAMM
Target/Specificity Human PGAM2
Reconstitution & Storage Store at -20°C. Minimize freezing and thawing.
PrecautionsPGAM2 Antibody (clone 4E9) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGAM2
Synonyms PGAMM
Function Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
Tissue Location Expressed in the heart and muscle. Not found in the liver and brain.
Volume 50 µl
Citations (0)
citation

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Background

Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.

References

Castella-Escola J.,et al.Gene 91:225-232(1990).
Tsujino S.,et al.J. Biol. Chem. 264:15334-15337(1989).
Shanske S.,et al.J. Biol. Chem. 262:14612-14617(1987).
Denis N.J.,et al.Proteomics 7:868-874(2007).
Tsujino S.,et al.Am. J. Hum. Genet. 52:472-477(1993).

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Discontinued
Cat# ALS15839
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