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AFAP1 / AFAP Antibody (N-Terminus)

Rabbit Polyclonal Antibody

     
  • IHC - AFAP1 / AFAP Antibody (N-Terminus) ALS15877
    Anti-AFAP1 / AFAP antibody IHC staining of human brain, cortex.
  • WB - AFAP1 / AFAP Antibody (N-Terminus) ALS15877
    Western blot analysis of AFAP1 in HeLa cell lysate with AFAP1 antibody at (A) 1 and (B) 2 ug/ml.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q8N556
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 81kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 60312
Other Names Actin filament-associated protein 1, 110 kDa actin filament-associated protein, AFAP-110, AFAP1, AFAP
Target/Specificity Human AFAP1. Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsAFAP1 / AFAP Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AFAP1
Synonyms AFAP
Function Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Cellular Location Cytoplasm, cytoskeleton. Note=Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction
Tissue Location Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF- 7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
Research Areas
Citations (0)

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Background

Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.

References

Han B.,et al.J. Biol. Chem. 279:54793-54801(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Olsen J.V.,et al.Cell 127:635-648(2006).

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$ 395.00
Cat# ALS15877
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