|Application ||WB, IHC-P, IF, E|
|Reactivity||Human, Mouse, Rat|
|Dilution||IF (20 µg/ml), IHC-P (5 µg/ml), WB (1-2 µg/ml)|
|Other Names||Rap guanine nucleotide exchange factor 4, Exchange factor directly activated by cAMP 2, Exchange protein directly activated by cAMP 2, EPAC 2, cAMP-regulated guanine nucleotide exchange factor II, cAMP-GEFII, RAPGEF4, CGEF2, EPAC2|
|Target/Specificity||Human RAPGEF4 / EPAC2. At least two isoforms of EPAC2 are known to exist; this antibody will detect only the larger isoform.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||RAPGEF4 / EPAC2 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA- independent exocytosis through interaction with RIMS2 (By similarity).|
|Cellular Location||Cytoplasm. Membrane; Peripheral membrane protein|
|Tissue Location||Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels|
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Provided below are standard protocols that you may find useful for product applications.
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA- independent exocytosis through interaction with RIMS2 (By similarity).
Kawasaki H.,et al.Science 282:2275-2279(1998).
Ueno H.,et al.Genomics 78:91-98(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
de Rooij J.,et al.J. Biol. Chem. 275:20829-20836(2000).
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