|Application ||WB, IHC-P, IF, E|
|Dilution||IF (5 µg/ml), IHC-P (10 µg/ml), WB (1 µg/ml)|
|Other Names||Periphilin-1, Gastric cancer antigen Ga50, PPHLN1|
|Target/Specificity||Human PPHLN1 / Periphilin.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||PPHLN1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416). Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697). May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (Probable).|
|Cellular Location||Nucleus Cytoplasm. Chromosome. Note=In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457). Localizes to chromatin (PubMed:26022416).|
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Provided below are standard protocols that you may find useful for product applications.
Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Line A.,et al.Br. J. Cancer 86:1824-1830(2002).
Kazerounian S.,et al.J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Li W.B.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
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