|Application ||WB, IHC-P, IF, E|
|Dilution||IF (5 µg/ml), IHC-P (10 µg/ml), WB (1 µg/ml)|
|Other Names||Periphilin-1, Gastric cancer antigen Ga50, PPHLN1|
|Target/Specificity||Human PPHLN1 / Periphilin.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||PPHLN1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416). Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697). May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (Probable).|
|Cellular Location||Nucleus Cytoplasm. Chromosome. Note=In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457). Localizes to chromatin (PubMed:26022416).|
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Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Line A.,et al.Br. J. Cancer 86:1824-1830(2002).
Kazerounian S.,et al.J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Li W.B.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
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