|Application ||WB, IHC-P, IHC-Fr|
|Dilution||IHC-Fr (1:200), IHC-P (1:50), WB (1:1000)|
|Other Names||78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78|
|Target/Specificity||Recognizes endogenous levels of total human BiP protein at 78kD. Species cross-reactivity: mouse.|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||HSPA5 / GRP78 / BiP Antibody (Gly584) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.|
|Cellular Location||Endoplasmic reticulum lumen. Melanosome. Cytoplasm Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
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Provided below are standard protocols that you may find useful for product applications.
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Ting J.,et al.DNA 7:275-286(1988).
Chao C.C.K.,et al.Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
Hansen J.J.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Bermudez-Fajardo A.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).
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