|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:40000), IHC-P (10 µg/ml), WB (1:500-1:1000)|
|Other Names||Nesprin-3, Nuclear envelope spectrin repeat protein 3, SYNE3, C14orf49|
|Target/Specificity||C14orf49 Antibody detects endogenous levels of total C14orf49 protein.|
|Reconstitution & Storage||Store at -20°C for up to one year.|
|Precautions||SYNE3 / C14orf49 Antibody (aa169-218) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.|
|Cellular Location||Nucleus outer membrane; Single-pass type IV membrane protein. Nucleus envelope. Rough endoplasmic reticulum|
|Tissue Location||Expressed in aortic endothelial cells (at protein level).|
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Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow- induced centrosome polarization and directional migration in aortic endothelial cells.
Li W.B.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
Heilig R.,et al.Nature 421:601-607(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Wilhelmsen K.,et al.J. Cell Biol. 171:799-810(2005).
Stewart-Hutchinson P.J.,et al.Exp. Cell Res. 314:1892-1905(2008).
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