|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:5000), IHC-P (20 µg/ml), WB (1:500-1:1000)|
|Other Names||Cyclin-G-associated kinase, 220.127.116.11, GAK|
|Target/Specificity||GAK Antibody detects endogenous levels of total GAK protein.|
|Reconstitution & Storage||Store at -20°C for up to one year.|
|Precautions||GAK Antibody (aa101-150) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.|
|Cellular Location||Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions|
|Tissue Location||Ubiquitous. Highest in testis.|
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Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Kimura S.H.,et al.Genomics 44:179-187(1997).
Greener T.,et al.J. Biol. Chem. 275:1365-1370(2000).
Daub H.,et al.Mol. Cell 31:438-448(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).
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