CD22 Antibody (clone 1B1)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF |
---|---|
Primary Accession | P20273 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 1B1 |
Calculated MW | 95kDa |
Dilution | IF (1:100), IHC-P (5 µg/ml), WB (1:4000) |
Gene ID | 933 |
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Other Names | B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, CD22, CD22, SIGLEC2 |
Target/Specificity | Human CD22 |
Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
Precautions | CD22 Antibody (clone 1B1) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CD22 {ECO:0000303|PubMed:1691828, ECO:0000312|HGNC:HGNC:1643} |
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Function | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6- linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. |
Cellular Location | Cell membrane; Single-pass type I membrane protein |
Tissue Location | B-lymphocytes. |
Volume | 50 µl |
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Background
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
References
Stamenkovic I.,et al.Nature 345:74-77(1990).
Wilson G.L.,et al.J. Exp. Med. 173:137-146(1991).
Wilson G.L.,et al.J. Immunol. 150:5013-5024(1993).
Suzuki Y.,et al.Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
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