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HAND1 Antibody (clone 1G10)

Mouse Monoclonal Antibody

     
  • WB - HAND1 Antibody (clone 1G10) ALS15986
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1...
  • IF - HAND1 Antibody (clone 1G10) ALS15986
    Anti-HAND1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently...
  • IHC - HAND1 Antibody (clone 1G10) ALS15986
    Anti-HAND1 antibody IHC staining of human kidney.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, Flo
Primary Accession O96004
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1G10
Calculated MW 24kDa
Dilution Flo (1:100), IF (1:100), IHC-P (10 µg/ml), WB (1:500) ,
Additional Information
Gene ID 9421
Other Names Heart- and neural crest derivatives-expressed protein 1, Class A basic helix-loop-helix protein 27, bHLHa27, Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1, eHAND, HAND1, BHLHA27, EHAND
Target/Specificity Human HAND1
Reconstitution & Storage Store at -20°C. Minimize freezing and thawing.
PrecautionsHAND1 Antibody (clone 1G10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HAND1
Synonyms BHLHA27, EHAND
Function Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box) (By similarity).
Cellular Location Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity).
Tissue Location Heart.
Research Areas
Citations (0)

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Background

Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box) (By similarity).

References

Knoefler M.,et al.Gene 224:77-86(1998).

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$ 395.00
Cat# ALS15986
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