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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RICK / RIP2 Antibody (clone 7F5)   

RICK / RIP2 Antibody (clone 7F5)

Mouse Monoclonal Antibody

     
  • IHC - RICK / RIP2 Antibody (clone 7F5) ALS15997
    IHC with RIPK2 antibody on frozen human pancreas.
  • WB - RICK / RIP2 Antibody (clone 7F5) ALS15997
    RIPK2 monoclonal antibody, clone 7F5 Western blot of RIP2 expression in HeLa.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IHC-Fr
Primary Accession O43353
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7F5
Calculated MW 61kDa
Dilution IHC-Fr (1:50)
Additional Information
Gene ID 8767
Other Names Receptor-interacting serine/threonine-protein kinase 2, 2.7.11.1, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, RIP-2, Tyrosine-protein kinase RIPK2, 2.7.10.2, RIPK2, CARDIAK, RICK, RIP2
Target/Specificity Human RIPK2
Reconstitution & Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRICK / RIP2 Antibody (clone 7F5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RIPK2
Synonyms CARDIAK, RICK, RIP2
Function Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
Cellular Location Cytoplasm.
Tissue Location Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node
Research Areas
Citations (0)

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Background

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

References

Inohara N.,et al.J. Biol. Chem. 273:12296-12300(1998).
McCarthy J.V.,et al.J. Biol. Chem. 273:16968-16975(1998).
Thome M.,et al.Curr. Biol. 8:885-888(1998).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 450.00
Cat# ALS15997
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