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>   home   >   Products   >   Primary Antibodies   >   FHIT Antibody (C-Terminus)   

FHIT Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • WB - FHIT Antibody (C-Terminus) ALS16032
    Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 ug/ml.
  • IF - FHIT Antibody (C-Terminus) ALS16032
    Immunofluorescence of FHIT in HeLa cells with FHIT antibody at 5 ug/ml.
  • IHC - FHIT Antibody (C-Terminus) ALS16032
    Anti-FHIT antibody IHC staining of human kidney.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P49789
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 17kDa
Dilution IF (5 µg/ml), IHC-P (10 µg/ml), WB (1-2 µg/ml)
Additional Information
Gene ID 2272
Other Names Bis(5'-adenosyl)-triphosphatase, 3.6.1.29, AP3A hydrolase, AP3Aase, Diadenosine 5', 5'''-P1, P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FHIT
Target/Specificity FHIT antibody is human and mouse reactive.
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsFHIT Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FHIT
Function Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
Cellular Location Cytoplasm. Mitochondrion. Nucleus.
Tissue Location Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines
Citations (0)

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Background

Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.

References

Ohta M.,et al.Cell 84:587-597(1996).
Druck T.,et al.Cancer Res. 57:504-512(1997).
Corominas R.,et al.Nat. Commun. 5:3650-3650(2014).
Naqvi S.R.A.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS16032
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