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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   DNM1L / DRP1 Antibody (Internal)   

DNM1L / DRP1 Antibody (Internal)

Rabbit Polyclonal Antibody

     
  • ICC - DNM1L / DRP1 Antibody (Internal) ALS16048
    Immunocytochemistry of DNM1L in HeLa cells with DNM1L antibody at 5 µg/mL.
  • IF - DNM1L / DRP1 Antibody (Internal) ALS16048
    Immunofluorescence of DNM1L in HeLa cells with DNM1L antibody at 20 µg/mL.
  • IHC - DNM1L / DRP1 Antibody (Internal) ALS16048
    Anti-DNM1L / DRP1 antibody IHC staining of human colon.
  • WB - DNM1L / DRP1 Antibody (Internal) ALS16048
    Western blot analysis of DNM1L in HeLa cell lysate with DNM1L antibody at 1 ug/ml.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E
Primary Accession O00429
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 82kDa
Dilution ICC (5 µg/ml), IF (20 µg/ml), IHC-P (10 µg/ml), WB (1-2 µg/ml) ,
Additional Information
Gene ID 10059
Other Names Dynamin-1-like protein, 3.6.5.5, Dnm1p/Vps1p-like protein, DVLP, Dynamin family member proline-rich carboxyl-terminal domain less, Dymple, Dynamin-like protein, Dynamin-like protein 4, Dynamin-like protein IV, HdynIV, Dynamin-related protein 1, DNM1L, DLP1, DRP1
Target/Specificity DNM1L antibody is human and mouse reactive. At least four isoforms of DNM1L are known to exist; this antibody will detect the two longest isoforms.
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsDNM1L / DRP1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNM1L
Synonyms DLP1, DRP1
Function Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane- associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis (PubMed:26992161). Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution.
Cellular Location Cytoplasm, cytosol. Golgi apparatus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein Peroxisome. Membrane, clathrin-coated pit Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Note=Mainly cytosolic. Translocated to the mitochondrial membrane through O-GlcNAcylation and interaction with FIS1. Recruited to the mitochondrial outer membrane by interaction with MIEF1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division Localizes to mitochondria following necrosis induction. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex. Binds to phospholipid membranes
Tissue Location Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
Research Areas
Citations (0)

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Background

Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane- associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Also required for mitochondrial fission during mitosis. Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution.

References

Shin H.-W.,et al.J. Biochem. 122:525-530(1997).
Hong Y.-R.,et al.Biochem. Biophys. Res. Commun. 249:697-703(1998).
Imoto M.,et al.J. Cell Sci. 111:1341-1349(1998).
Chen C.-H.,et al.DNA Cell Biol. 19:189-194(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS16048
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